ABT Equine Vitrification Kit

This Vitrification Kit helps with the process for freezing equine embryos. The process of equine embryo vitrification allows the horse embryos to survive the freezing and thawing process. When using the enclosed material for long-term storage of vitrified equine embryos, it is essential to know the age and diameter of the embryos.

This Vit Kit for the embryo transfer for horses includes 1 x 8 ml vial of each: VS1 - Vitrification Solution 1, VS2 - Vitrification Solution 2, VS3 - Vitrification Solution 3 and diluent. When it comes to how to store equine embryos with a vit kit, it is highly recommended that embryos be 6 to 6.5 days of age (day 0 = day of ovulation), or approximately 8 days after administration of hCG. The ideal embryo stage for vitrification is a morula or early blastocyst. Ideally, the outer zona of the embryos should be less than 300 microns in diameter and, in the case of blastocysts, the zona should be no less than 1 /2 the normal thickness. It is highly recommended that an optical micrometer be used to measure the diameter of embryos to be vitrified. When performing an embryo transplant in horses with this Vit Kit, embryos with an outer diameter of more than 300 microns will have a very low survival rate following vitrification.

The absolute number that can be vitrified from one kit is partially dependent on how many are done in the same day. If practitioners take only 0.5 ml of the diluent out and only do one embryo per day, then the kit would do just 10 embryos. More than that would be possible if more than one is vitrified the same day or if they take out less diluent. Only 0.18 ml of diluent is needed per straw, so taking out about 0.25 ml is likely sufficient.

Equine Vitrification Kit Insert

This Vit Kit is based on technology developed by the Animal Reproduction and Biotechnology Laboratory at Colorado State University, Ft. Collins, Colorado.