- With the Fungassary® Dermatophyte Test Kit, it's easy to diagnose fungus infections on the skin, including ringworm
- Place the infected hair or scales on the test medium and medium color will change to indicate diagnosis
- Color will change as early as 48 hours after inoculation
- No preparation necessary
- For Veterinary Use
Destroy the used test medium bottle by incineration to eliminate the spread of all organisms.
When removing the cap from the bottle, take care to avoid contamination.
When replacing the cap, make certain that it remains loose so that air exchange can occur in the bottle during incubation. When the cap is too tight, color change will not develop.
Keep out of reach of children.
2. Select hair and scales for culture from both the periphery and the center of the lesion. Broken, frayed or distorted hairs, and those that fluoresce with Wood's light, are the best specimens.
3. Remove only a small portion of hair and scales from the lesion with a hemostat or thumb forcep. Avoid placing large amounts of hair and scales on the medium: this will produce useless overgrowth of the contaminants.
4. When removing the cap from the test medium bottle, take care to avoid contamination.
5. Press the hair and scales on the culture medium to ensure good contact, but do not bury the specimen in the medium.
6. When replacing the cap, make certain that it remains loose so that air exchange can occur in the bottle during incubation. When the cap is too tight color change will not develop.
7. Identify the bottle with patient and date.
8. Incubate at room temperature so that suitable growth will occur.
9. Evaluation of the test results can begin as early as 48 hours after inoculation. A pinkish color will appear in the amber medium under the specimen and developing colony. The color will intensify as growth proceeds and is due to alkaline metabolites produced by the dermatophytes. When a positive dermatophyte infection is present, the entire medium will turn red by the seventh to fourteenth day. If there is no growth within ten days, redistribute the sample on the medium. Occasionally growth does not occur because of improper inoculation.
10. A color change may occasionally be produced by a specimen heavily contaminated with saprophytic fungi or bacteria. However this is not a problem because differentiation from dermatophytes can be made as follows:
Dermatophyte: A color change appears in the medium with colony growth - colony pigments are usually light colored.
Saprophyte fungi: Colony growth is well established before any color change appears in the medium - colony pigments are usually dark colored.
Bacteria: The morphology of bacterial colonies differs from the morphology of fungal colonies.