- Maximum protection for horse sperm transport
- Contains cholesterol to increase stability
- For stallion sperm more sensitive to cooled transport
- Use during centrifugation in freezing protocols
- For equine reproduction and breeding
Equine semen extender based on skimmed milk with the addition of cholesterol. Cholesterol is a component of the spermatic membrane that contributes to an increase in stability. Botusemen Special is highly recommended for pre-freezing centrifugation.
For fresh or cooled equine semen for horse breeding. Ideal for stallions especially sensitive to cooled transport.
The temperature of 5° C or 15° C can be used for semen cooled transport as well as for pre-freezing centrifugation. Use dilutions of 30 to 50 million/ml for cooled transport, respecting the minimum of 2 parts of extender to 1 part of semen.
In case of samples requiring centrifugation before cooled transport should be respected the resuspension of spermatozoa to 100 million/ml. DO NOT exceed the appropriate concentrations of cholesterol.
Storage: After mixing the contents of the sachet with sterile water, store it at 2 to 8°C (35.6 to 46.4°F) for up to 3 days.
How to use Botusemen Special: The minimum dilution rate for preserving sperm motility during storage is 2 parts extender to 1 part semen, aiming for a ﬁnal sperm concentration of 30 to 50 million total sperm per mL.
Important: In cases where centrifugation before the cooling process is necessary, resuspend the sperm pellet at a ﬁnal concentration of 100 million total sperm per mL.
100 mg gentamicin and 100 mg penicillin per 100 ml.