By measuring the amount of relaxin in the blood, the WITNESS® Relaxin Test Kit is able to distinguish between pseudo pregnancy and gestation in dogs and cats. This test gives a reliable assessment of pregnancy status. The test detects relaxin produced by the developing placenta(s) as early as day 20 after the luteinizing hormone surge. Relaxin is secreted primarily by the placenta, and it is the only hormone specifically associated with canine and feline pregnancy. The presence of significant amounts of relaxin is a reliable indicator of pregnancy. A sudden decrease in relaxin may indicate that abortion or spontaneous abortion has occurred. Requires only a small sample of serum or plasma; whole blood cannot be used. Gives results within 10 minutes. Kit includes 5 tests, 1 buffer bottle, 5 pipettes and instructions. A centrifuge is needed to get the serum and plasma required for the test.
It is recommended to test samples immediately after collection. If samples are kept at room temperature, they should be tested within 4 hours. Otherwise, samples should be refrigerated at 2-7°C (35-45°F) and may be held for up to 48 hours.
Do not use components after expiration date.
Refrigeration not required. Store the test kit at 2-25°C (35-77°F). Do not freeze.
Use the test within 10 minutes after opening the sealed pouch.
Use a separate pipette for each sample.
Important: Allow sample and buffer drops to fall onto membrane at window #1. Do not touch pipette tip, sample drops, buffer drops, or buffer bottle tip directly to the membrane.
1. Sample application: Tear open the pouch provided and place the test on a flat surface for the duration of the test. Squeeze the provided pipette near the sealed end. Insert the pipette into the serum or plasma sample and draw up a small amount. Holding the pipette vertically, transfer two drops into window #1.
2. Buffer dispensing: Remove the cap from the buffer bottle, hold it vertically and add two drops of buffer into window #1.
3. Reading test: Wait 10 minutes, and then observe the presence or absence of pink/purple bands in reading windows #2 (sample results) and #3 (control).
Validation: Test is validated if a pink/purple band is present in the reading window #3.
Negative for relaxin: No band in reading window #2, with one pink/purple band in window #3. Any negative result is considered indicative of non-pregnancy.
Positive for relaxin: One pink/purple band in reading window #2, with one pink/purple band in window #3. A positive result is considered indicative of pregnancy. A weak positive result may be observed at the beginning of pregnancy.
It is recommended to repeat the test on a fresh sample to confirm negative or positive results.
For use with dogs and cats.
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