VetScan Canine Parvovirus Rapid Test
Item #: 22922-155
- VetScan® Canine Parvovirus Rapid Test is used to detect parvo antigens in canine feces
- Unique formula gives great specificity and sensitivity
- Accurate results within 10 minutes
- Room temperature storage (59 - 80°F)
- For veterinary use only
- Test Kits are non-returnable
VetScan® Canine Parvovirus Rapid Test provides accurate results within 10 minutes. It uses a unique formula that gives the test great specificity and sensitivity for the detection of parvovirus antigens in canine feces. Simply coat the swab with the fecal sample and break the seal to mix with diluents. Dispense three drops of specimen into the sample well and read results. Room temperature storage (59 - 80°F). Do not freeze. No returns on test kits. For veterinary use only.
10 Test Devices (Each sealed pound contains a test strip in a plastic cassette and a desiccant.)
10 Sample dilution devices (Each consists of a sampling swab and 0.7 ml of Extraction Buffer with sodium azide (0.02%) as a preservative.
1 instruction insert
The test kit is to be stored at room temperature (15° to 27°C (59° to 80°F) for the duration of the shelf life. Avoid the test kit being subjected to excessive heat or freezing for a long period of time.
Fresh samples should be used when possible. If samples cannot be used within 24 hours, they should be stored cold (2°-8°C or 36°-46°F) for up to two weeks, or kept frozen before being tested.
Some fecal samples contain mucous. In order to collect an accurate sample, avoid coating the swab completely with mucous material.
Remove the sampling swab from the dilution chamber of the Sample Dilution Device. Coat the sampling swab with a thin layer of fecal material or saturate the swab if the sample is liquid. Return the sampling swab back into the dilution chamber.
Break the seal of the Extraction Buffer storage bulb located on the top of the Sample Dilution Device by bending the bulb back and forth until the internal blue stick breaks at its base. Squeeze the bulb 5 - 6 times to thoroughly mix the sample with the Extraction Buffer.
Remove a Test Device from the protective pouch and place it on a flat surface.
Using the buffer storage bulb and the sampling swab as a pipette, transfer 3 drops of the diluted sample into the round sample well of the Test Device. Hold the pipette vertically to ensure proper sample volume. Start timing.
Read the results between 10 minutes and 15 minutes. Strong positive results may appear as soon as 1 minute, and weak positive results may take up to 10 minutes to appear. After 15 minutes, discard the Test Device because the results are no longer valid.
The result is negative when one red to pink line appears in the C (control) area.
The result is positive when two red to pink lines appear. One red/pink line will appear in the T (test) area and one in the C (control) area. A red to pink line with any intensity in the T (test) area should be considered positive. Colored lines may be lighter or darker than each other.
The test is invalid when no colored line appears in the C (control) area, even if a red to pink line appears in the T (test) area. Repeat the test using a new cassette. Colored lines that appear after 15 minutes are not diagnostic and should be ignored.
Sensitivity and Specificity
In an USDA approved study involving 258 samples, the VetScan Canine Parvovirus Rapid Test correctly identified 93/96 hemagglutination inhibition (HI) positive canine fecal samples and 157/162 negative canine fecal samples. The discrepant results were resolved by PCR. The sensitivity and the specificity of the test was 96.9% and 96.9%, respectively.
Precautions and Warnings:
Parvovirus is infectious. Samples should be handled accordingly. The user should refer to the relevant section of the CDC-OHS manual “Biosafety in Microbiology and Biomedical Laboratories,” 5th Edition, 2007 for handling and disposal of the specimens, devices, and reagent.
Extraction buffer contains sodium azide, which may react with lead and copper plumbing to form explosive metal azides. Azide build-up may be avoided by flushing drains with large volumes of water after disposal.
The Test Device should remain in the sealed pouch until ready for use.
The Test Device and the Sample Dilution Device should be used when they are at room temperature (15° to 27°C (59° to 80°F)
Do not use kit or materials beyond expiration date.
Do not mix or interchange serials of VetScan Canine Parvovirus Rapid Test devices.
Use a new Test Device and a new Sample Dilution Device for each testing. Do not reuse devices.