The Snap® Parvo Test Kit can detect the parvovirus antigen in canine feces, without cross-reacting with the modified-live vaccine even after vaccination. Delivers accurate results within eight minutes. The color development on the sample spot indicates the presence of the antigen, while the negative control spot helps safeguard against false positives and determines invalid test results. Room temperature storage (35 - 77°F). For veterinary use only. Made in USA. No returns on test kits.
5 swabs with antiparvovirus: HRPO conjugate
5 Snap® devices (Each Snap® device contains 0.4 ml of wash solution and 0.6 ml of substrate solution.)
1 instruction insert
The test kit is to be stored at room temperature 2° to 25°C (35° to 77°F) until the expiration date.
All components must be at room temperature 18-25°C (64°-77°F) before running the test. Do not heat. This will take at least 30 minutes depending upon the temperature of your laboratory.
Samples must be at room temperature 18-25°C (64°-77°F) before beginning the test procedure.
Canine fecal matter is required for this test. Swabs are provided for sampling.
Fecal samples can be stored at 2-8°C (35-46°F) for 24 hours. If longer storage is required, samples should be frozen.
1. If stored in a refrigerator, allow all components to equilibrate at room temperature 18-25°C (64-77°F) for 30 minutes. Do not heat.
2. Obtain a sampling swab and a Snap® device for each sample to be tested. Pull and twist the tube covering the swab tip to remove the tube from the swab/reagent bulb assembly (A). Using the swab, coat the swab tip with fecal material. Then, return the swab to the tube (B).
Note: Only a thin coat of fecal material on the swab is required; do not coat the swab with excess feces.
3. Break the purple valve stem inside the bulb assembly by bending the assembly at the narrow neck (C). (Re-bending the opposite way may be helpful.) Squeeze the reagent bulb three times to pass the blue solution through the swab tip and mix it with the sample (D).
4. Place the Snap® device on a horizontal surface. Using the swab as a pipette, dispense drops of the fluid into the sample well, being careful not to splash contents outside of the sample well. The sample will flow across the result window, reaching the activation circle in 30-60 seconds. Some sample may remain in the sample well.
5. When color FIRST appears in the activation circle, push the activator firmly until it is flush with the device body.
Note: Some samples may not flow to the activation circle within 60 seconds, and, therefore, the circle may not turn color. In this case, press the activator after the sample has flowed across the result window.
6. Read the test result at 8 minutes.
Interpreting the Test Results
To determine the test result, read the reaction spots in the result window and compare the color intensity of the sample spot to that of the negative control spot.
Color development in the sample spots that is darker than the negative control indicates a positive result, and the presence of parvovirus antigen in the sample.
Color development only in the positive control spot indicates a negative result.
Negative control (safeguard against false-positives) If color in the negative control spot is equal to or darker than the color in the sample spot, the result is invalid and the sample should be retested.
No color development If the positive control does not develop color, repeat the test.
Background If the sample is allowed to flow past the activation circle, background color may result. Some background color is normal. However, if colored background obscures the test result, repeat the test.
For the detection of canine parvovirus (CPV) antigen in canine feces.